This unique sand-mediated cryopreservation method may greatly facilitate the convenient and ready availability of high-quality hiPSCs and probably many other types of cells/tissues for the emerging cell-based translational medicine. Furthermore, the cryopreserved hiPSCs retain high pluripotency and functions judged by their pluripotency marker expression, cell cycle analysis, and capability of differentiation into the three germ layers. Here, sand from nature is discovered to be capable of seeding ice above −10 ☌, which enables cryopreservation of hiPSCs with no serum, much-reduced cryoprotectant, and high cell survival. The latter may cause spontaneous differentiation and/or introduce xenogeneic factors, which may compromise the quality of hiPSCs. Most of the annotations are declarative but you can also include C code fragments that are dynamically compiled. Microsoft Bot Builder extension for specifying a guided FormFlow dialog using an extended JSON Schema. However, contemporary methods for hiPSC cryopreservation are associated with both limited cell survival and high concentration of toxic cryoprotectants and/or serum. Formflow Download This is an unofficial.NET Core 2.0/.NET Framework 4.5 ported version of .Json package. Human induced pluripotent stem cells (hiPSCs) possess tremendous potential for tissue regeneration and banking hiPSCs by cryopreservation for their ready availability is crucial to their widespread use.
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